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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind especially into the goal antibody and block antibody binding. These peptide generally has the epitope recognized by the antibody.
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This information outlines even further feasible optimization of mobile and immunoassay parameters to be certain the absolute best effects are...
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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to be sure the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope thr77 login to the focus on protein. By comparing the staining from your blocked antibody vs . the antibody by itself, one can see which staining is distinct.
Concentrate on Info Cyclin E belongs on the extremely conserved cyclin family, whose members are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead into the temporal coordination of each and every mitotic party. Cyclin E types a complex with and functions like a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S changeover. This protein accumulates at the G1-S period boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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